| Autor: | Hiroshi Ohshima, Brigitte Pignatelli, Chun-Qi Li |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Physiology
Nitrotyrosine Gastroenterology Chronic gastritis Inflammation Biology Helicobacter pylori bacterial infections and mycoses medicine.disease_cause biology.organism_classification medicine.disease Molecular biology digestive system diseases Pathogenesis chemistry.chemical_compound chemistry Immunology medicine CagA Gastritis medicine.symptom Oxidative stress |
| Zdroj: | Digestive Diseases and Sciences. 46:836-844 |
| ISSN: | 0163-2116 |
| DOI: | 10.1023/a:1010764720524 |
| Popis: | In order to study the role of Helicobacter pylori infection in gastric carcinogenesis, we have measured oxidized (carbonyls) and nitrated (nitrotyrosine-containing) proteins as markers for oxidative and nitrative stress in 216 human gastric biopsies using dot and western immunoblots and correlated the results with H. pylori, cagA status, expression of interleukin-8 and inducible nitric oxide synthase (iNOS) mRNAs, and gastric pathology. Higher levels of both oxidized and nitrated proteins were found in patients with either chronic gastritis or duodenal ulcer than in those with normal mucosa. The levels of modified proteins were significantly higher in inflamed samples infected with H. pylori, especially cagA+ strains, and in those with expression of interleukin-8 and iNOS mRNAs than in those negative for these parameters. These results indicate that infection with cagA+ H. pylori induces significant oxidative and nitrative stress in stomach mucosa, contributing to the pathogenesis of H. pylori-associated gastroduodenal diseases. |
| Databáze: | OpenAIRE |
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