Redox metabolic imaging of NASH model mice using in vivo dynamic nuclear polarization MRI

Autor: Hinako Eto, Masayuki Matsuo, Tatsuya Naganuma, Makoto Hashizume, Tomoko Nakaji, Fuminori Hyodo, Masaharu Murata, Ryosuke Nakata
Rok vydání: 2018
Předmět:
Zdroj: Free Radical Biology and Medicine. 128:S86
ISSN: 0891-5849
DOI: 10.1016/j.freeradbiomed.2018.10.197
Popis: As a consequence of the global epidemic of metabolic syndrome and chronic obesity, non-alcoholic fatty liver disease (NAFLD) has emerged as an important contribution in several chronic liver diseases. The development of a non-invasive diagnostic method for assessing disease progression from NAFLD to non-alcoholic steatohepatitis (NASH) has become an important research goal. Oxidative stress is thought to play a central role in the progression of NAFLD to NASH. Mitochondria are involved in both ROS generation, and free fatty acid (FFA) s-oxidation that promotes lipid accumulation. Currently available non-invasive imaging technologies are only able to assess fat accumulation in the liver. Therefore, these methods are not suitable for a precise diagnosis of NASH. The standard diagnostic technique for NASH, liver biopsy, has several drawbacks, including the higher risk of complications that accompanies invasive procedures. Here, we demonstrated that in vivo mitochondrial redox metabolism was dramatically altered at an early stage, before histopathological changes, and NASH could be accurately diagnosed by in vivo dynamic nuclear polarization-magnetic resonance imaging in methionine-choline-deficient (MCD) diet-induced NASH mouse model. In addition, this technique was feasible for the diagnosis of NASH compared with histopathological findings from biopsies. Our data reveal a novel method for monitoring the dynamics of redox metabolic changes in NAFLD/NASH.
Databáze: OpenAIRE
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