Hepatic Reduction of Carbamoyl-PROXYL in Ferric Nitrilotriacetate Induced Iron Overloaded Mice: An in Vivo ESR Study
| Autor: | Yumiko Yamaguchi, Noppawan Phumala Morales, Kimiyo Murakami, Nuttavut Kosem, Hideo Utsumi |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Pharmacology
medicine.medical_specialty Antioxidant Reducing agent medicine.medical_treatment Pharmaceutical Science General Medicine Glutathione medicine.disease_cause Deferoxamine Lipid peroxidation chemistry.chemical_compound Endocrinology chemistry Biochemistry In vivo Internal medicine medicine Carcinogen Oxidative stress medicine.drug |
| Zdroj: | Biological and Pharmaceutical Bulletin. 35:1035-1040 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.b110701 |
| Popis: | Reduction of a nitroxyl radical, carbamoyl-PROXYL in association of free radical production and hepatic glutathione (GSH) was investigated in iron overloaded mice using an in vivo L-band electron spin resonance (ESR) spectrometer. Significant increases in hepatic iron, lipid peroxidation and decrease in hepatic GSH were observed in mice intraperitoneally (i.p.) administrated with ferric nitrilotriacetate (Fe(III)-NTA, a total 45 µmol/mouse over a period of 3 weeks). Free radical production in iron overloaded mice was evidenced by significantly enhanced rate constant of ESR signal decay of carbamoyl-PROXYL, which was slightly reduced by treatment with iron chelator, deferoxamine. Moreover, the rate constant of ESR signal decay was negatively correlated with hepatic GSH level (r=-0.586, p 80%) in mice through daily i.p. injection and drinking water supplementation of L-buthionine-[S,R]-sulfoximine (BSO) significantly retarded ESR signal decay, while there were no changes in serum aspartate aminotransferase and liver thiobarbituric acid-reactive substances levels. In conclusion, GSH plays two distinguish roles on ESR signal decay of carbamoyl-PROXYL, as an antioxidant and as a reducing agent, dependently on its concentration. Therefore, it should be taken into account in the interpretation of free radical production in each specific experimental setting. |
| Databáze: | OpenAIRE |
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