Comparison of Methods for Determining Dead Times in Supercritical Fluid Chromatography
| Autor: | V. V. Lunin, O. O. Parenago, M. O. Kostenko, O. I. Pokrovskiy |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
010304 chemical physics Component (thermodynamics) Oxide Analytical chemistry chemistry.chemical_element Dead time 010402 general chemistry 01 natural sciences Nitrogen 0104 chemical sciences chemistry.chemical_compound chemistry Yield (chemistry) Phase (matter) 0103 physical sciences Supercritical fluid chromatography Polar Physical and Theoretical Chemistry |
| Zdroj: | Russian Journal of Physical Chemistry B. 13:1111-1116 |
| ISSN: | 1990-7923 1990-7931 |
| DOI: | 10.1134/s1990793119070145 |
| Popis: | —The three most common methods for measuring chromatographic dead time in supercritical fluid chromatography are tested. It is shown that at low concentrations of a polar cosolvent in the mobile phase, the use of the simplest “system peak” method leads to highly inaccurate results. With an increase in the percentage of the cosolvent to 10–15%, this method can be used for a rough estimation of dead times, especially for nonpolar stationary phases. The use of nitrogen(I) oxide as a marker of the dead time of an unretained component, on the contrary, is well suited in the case of using pure carbon dioxide as a mobile phase. The static method, based on other principles, can theoretically yield very accurate values for dead time, but it requires knowledge of the velocity profile of the mobile phase along the path of the entire chromatography. |
| Databáze: | OpenAIRE |
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