High Pressure Liquid Chromatographic Determination of Cholesterol in Foods

Autor: Alan J. Sheppard, David R. Newkirk
Rok vydání: 1981
Předmět:
Zdroj: Journal of AOAC INTERNATIONAL. 64:54-57
ISSN: 0004-5756
DOI: 10.1093/jaoac/64.1.54
Popis: A high pressure liquid chromatographic method that uses the benzoate ester of cholesterol has been developed for the measurement of cholesterol in foods. A 300 × 3.9 mm id μBondapak C18 column and a variable wavelength ultraviolet detector are used with a 100% methanol mobile phase. Amounts as low as 10 ng cholesterol benzoate can be detected. The results from the analyses of a variety of foods are given and compared with results from gas-liquid chromatographic analyses. The comparison of the 2 chromatographic techniques is favorable.
Databáze: OpenAIRE