Type 2 11β-Hydroxysteroid Dehydrogenase Activity in Human Fallopian Tube and Correlation of Enzyme Levels with Endometrial Histopathology
| Autor: | Sanaa Jalou, Vijaya L. Nacharaju, Monzila Rahman, Charles H. Bowers, Nezar Dalloul, Ozgul Muneyyirci-Delale, Allan Kessel, Lianfu Yang, Ovadia Abulafia |
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| Rok vydání: | 2005 |
| Předmět: |
endocrine system
medicine.medical_specialty animal structures urogenital system media_common.quotation_subject Immunology Obstetrics and Gynecology Biology Endometrium Enzyme assay Human fertilization Endocrinology medicine.anatomical_structure Reproductive Medicine Internal medicine medicine biology.protein Immunology and Allergy Histopathology Cortisone Menstrual cycle Glucocorticoid medicine.drug media_common Fallopian tube |
| Zdroj: | American Journal of Reproductive Immunology. 54:96-100 |
| ISSN: | 1046-7408 |
| DOI: | 10.1111/j.1600-0897.2005.00292.x |
| Popis: | PROBLEM The inter-conversion of hormonally active cortisol and inactive cortisone is catalyzed by 11beta-hydroxysteroid dehydrogenase (11beta-HSD). This conversion controls the level of active glucocorticoid concentration in tissues. As the fallopian tube plays a major role in the process of fertilization, we wanted to investigate whether 11beta-HSD is present in the human fallopian tube to control the glucocorticoid levels as in other tissues. METHOD OF STUDY Isthmic, ampullary and fimbrial portions of the fallopian tube are obtained from patients undergoing hysterectomy and salpingo-oopherectomy for symptomatic leomyomata uteri. 11beta-HSD activities were measured in the homogenates of the tube, cortisol as the steroid substrate. The enzyme activity was expressed as nanomolar cortisone formed per minute per gram of tissue (mean +/- S.D.). RESULTS A significant level of 11beta-HSD activity in oxidation direction was found in all three parts of the tube. There is no significant difference in the distribution of the enzyme activity throughout the tube. When tubal 11beta-HSD activity was compared with endometrial histology, the enzyme activity is significantly lower in proliferative endometrium when compared with secretory endometrium (P = 0.002). The enzyme activity in inactive endometrium is significantly higher than the active endometrium (P = 0.05). CONCLUSION The presence of 11beta-HSD throughout the fallopian tube and its correlation to endometrial histology is indicative of its probable role in controlling the glucocorticoid levels in the tissue, which in turn may influence the fertilization process. |
| Databáze: | OpenAIRE |
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