Identification of ecdysteroidogenic enzyme genes and their expression during pupal diapause in the cabbage armyworm,Mamestra brassicae

Autor:	Mari H. Ogihara, Kazuki Saito, Yoshinori Fujimoto, Juri Hikiba, Takayoshi Nakaoka, Yutaka Suzuki, H. Ikeda, Hiroshi Kataoka, Akira Mizoguchi, Nobuto Yamada
Rok vydání:	2017
Předmět:	0301 basic medicine medicine.medical_specialty Ecdysteroid animal structures fungi Biology Diapause Prothoracic gland Cell biology Pupa 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Endocrinology chemistry Insect Science Internal medicine Gene expression Genetics medicine Heterologous expression Molecular Biology Moulting Gene
Zdroj:	Insect Molecular Biology. 26:286-297
ISSN:	0962-1075
DOI:	10.1111/imb.12291
Popis:	In this study, we identified ecdysteroidogenic enzymes in the cabbage armyworm, Mamestra brassicae, and demonstrated reduced expression of these genes during diapause. Some insects employ a temporary developmental arrest, diapause, to survive in severe environments. The titres of the moulting hormone ecdysteroid were reduced in diapause pupae of M. brassicae; therefore, ecdysteroidogenesis might be suppressed by a diapause-specific mechanism. To clarify expression changes of ecdysteroidogenic enzyme genes during diapause in M. brassicae, we first identified the genes for seven ecdysteroidogenic enzymes: Neverland, Non-molting glossy (Nm-g), CYP307A1 (Spook), CYP306A1 (Phantom), CYP302A1 (Disembodied), CYP315A1 (Shadow) and CYP314A1 (Shade). Enzymatic assays using heterologous expression in Drosophila Schneider 2 (S2) cells and analysis of mRNA distribution indicated that the identified genes were ecdysteroidogenic enzymes of M. brassicae. Expression levels of these ecdysteroidogenic enzyme genes were compared between prothoracic glands in different pupal stages throughout diapause. Immediately after pupation, diapause-destined pupae showed similar expression levels of ecdysteroidogenic enzyme genes to those of nondiapause pupae. All of these genes showed reduced gene expression after diapause initiation. Expression was immediately increased in diapause-destined pupae at the postdiapause quiescence phase. These results indicate that reduced expression of ecdysteroidogenic enzyme genes suppresses ecdysteroidogenesis and maintains developmental arrest during diapause.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::326fd5a7a30b77bde91b47fb7d5522e3 https://doi.org/10.1111/imb.12291 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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