DEVELOPMENT AND VALIDATION OF A RAPID AND SIMPLE HPLC-UV METHOD FOR THE ANALYSIS OF SORAFENIB IN THE PRESENCE OF POLYAMIDOAMINE (PAMAM) DENDRIMERS

Autor:	Fakhrossadat Farvadi, Gh. Yousefi, Fatemeh Hashemi, Ali Mohammad Tamaddon
Rok vydání:	2014
Předmět:	Detection limit Isocratic elution Pamam dendrimers Chromatography Chemistry Calibration curve Clinical Biochemistry Cationic polymerization Pharmaceutical Science Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Dendrimer Acetonitrile
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 37:1427-1437
ISSN:	1520-572X 1082-6076
Popis:	A rapid and simple liquid chromatography-UV spectrometric assay was developed for the quantitative determination of sorafenib in the presence of cationic and anionic PAMAM dendrimers. PAMAM dendrimer:sorafenib (SFB) admixture was prepared by Higuchi rotating bottle method for different generations (G4, G4.5, G5). Separation was achieved on a C18 column (Knauer Eurospher 150 mm × 4.6 mm with precolumn) at room temperature using an isocratic elution method with acetonitrile/K-phosphate buffer 25 mM pH 7.4: 55/45 (v/v) at a flow rate of 1.5 ml/min. The SFB peak was separated well from PAMAM dendrimers peak. Calibration curves were linear in the concentration range of 0.097–100 mcg/mL with R2 > 0.9988. The accuracy [Bias (%)] and precision [RSD (%)] values, calculated from three different sets of quality control samples analyzed in triplicate on four different days, ranged from −3.6–16 and 2.1–14.5, respectively. The limit of quantitation was 97 ng/mL.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3260ecdae855e3477c10e0495c4bc486 https://doi.org/10.1080/10826076.2013.794737 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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