Chemical characterization and cytoprotective effect of the hydroethanol extract from Annona coriacea Mart. (Araticum)
| Autor: | José Geraldo de Alencar Santos Júnior, Janyketchuly S. Cristo, Ticiana C C Boris, Vandbergue Santos Pereira, Francisco Afrânio Cunha, Henrique Douglas Melo Coutinho, Maria Izabel Florindo Guedes, Najla M.N. Ferreira, Fernando G Figueiredo, Eleonora O Farina, Cícero M. G. Torres, Francisco José Cabral Mesquita, Polyana Amorim Cruz Nascimento, Gabriela Carneiro Teixeira, Ivon Teixeira de Souza, Vanderlan Nogueira Holanda, Sáskia Thamyles Bezerra Coutinho, Paulo H F Moreira, Pedro Everson Alexandre de Aquino, Nara Luana Ferreira Pereira |
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| Rok vydání: | 2016 |
| Předmět: |
Pharmacology
biology Traditional medicine Aminoglycoside biology.organism_classification Antimicrobial medicine.disease_cause 01 natural sciences High-performance liquid chromatography 0104 chemical sciences 010404 medicinal & biomolecular chemistry 03 medical and health sciences chemistry.chemical_compound Minimum inhibitory concentration 0302 clinical medicine chemistry Annona coriacea Staphylococcus aureus 030220 oncology & carcinogenesis Drug Discovery Botany medicine Quercetin Luteolin |
| Zdroj: | Pharmacognosy Research. 8:253 |
| ISSN: | 0974-8490 |
| DOI: | 10.4103/0974-8490.188882 |
| Popis: | Introduction: Annona coriacea Mart. (araticum) is a widely distributed tree in the cerrado. Its value is attributed principally to the consumption of its fruit which possesses a large nutritive potential. The objective was to identify the chemical profile and evaluate the antimicrobial and cytoprotective activity of the hydroethanol extract of A. coriacea Mart. (HEAC) leaves against the toxicity of mercury chloride. Materials and Methods: The characterization of components was carried out using high-performance liquid chromatography (HPLC). The minimum inhibitory concentration (MIC) was determined by microdilution method in broth with strains of Staphylococcus aureus , Escherichia coli , and Pseudomonas aeruginosa. For evaluation of the modulatory and cytoprotective activity of aminoglycoside antibiotics (gentamicin and amikacin) and mercury chloride (HgCl 2 ), the substances were associated with the HEAC at subinhibitory concentrations (MIC/8). Results and Discussion: The HPLC analysis revealed the presence of flavonoids such as Luteolin (1.84%) and Quercetin (1.19%) in elevated concentrations. The HEAC presented an MIC ≥512 μg/mL and significant antagonistic action in aminoglycosides modulation, and it also showed cytoprotective activity to S. aureus (significance P E. coli (significance P Conclusions: The results acquired in this study show that the HEAC presents cytoprotective activity over the tested strains in vitro and can also present antagonistic effect when associated with aminoglycosides, reinforcing the necessity of taking caution when combining natural and pharmaceutical products. |
| Databáze: | OpenAIRE |
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