Valorization of Activated Carbon as a Reusable Matrix for the Immobilization of Aspergillus oryzae Whole-Cells Expressing Fusarium heterosporum Lipase toward Biodiesel Synthesis
| Autor: | Shinji Hama, Emmanuel Quayson, Ayumi Yoshida, Jerome Amoah, Kenta Morita, Akihiko Kondo, Chiaki Ogino |
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| Rok vydání: | 2019 |
| Předmět: |
General Chemical Engineering
02 engineering and technology 010402 general chemistry 01 natural sciences chemistry.chemical_compound Aspergillus oryzae medicine Environmental Chemistry Organic chemistry Denaturation (biochemistry) Lipase chemistry.chemical_classification biology Renewable Energy Sustainability and the Environment Fatty acid General Chemistry Transesterification 021001 nanoscience & nanotechnology biology.organism_classification Cell aggregation 0104 chemical sciences chemistry biology.protein Methanol 0210 nano-technology Activated carbon medicine.drug |
| Zdroj: | ACS Sustainable Chemistry & Engineering. 7:5010-5017 |
| ISSN: | 2168-0485 |
| DOI: | 10.1021/acssuschemeng.8b05649 |
| Popis: | The biomass-derived source, low-cost and hydrophobicity/oleophilic advantages of activated carbon (AC) were explored for the immobilization of Aspergillus oryzae whole-cells expressing Fusarium heterosporum lipase. The adsorptive influence of AC favored growth of the cells into its porous interfaces with paralleled exterior dense film formation. Increasing AC weights hindered extracellular lipase activity. Cell aggregation of 0.34 ± 0.02 mg/BSP was found to be effective in catalyzing an industrially challenging feedstock (68.77% w/w free fatty acids, 20.48% w/w triglycerides) to 98% fatty acid methyl esters (FAME). In a comparative investigation with polyurethane as matrix, higher trans/esterification facilitation was observed with AC. Benefiting from the oleophilicity of AC; denaturation effect from methanol on the lipases was reduced. Surface characterization with FE-SEM, XPS and FT-IR evidenced effective cell-matrix adhesion and a retention of the AC’s intrinsic properties. The advantageous tribology o... |
| Databáze: | OpenAIRE |
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