Validation of the LacTek Test Applied to Spiked Extracts of Tissue Samples: Determination of Performance Characteristics

Autor:	Mansell W Griffiths, William B McNab, A. J. Yee, Scott A. McEwen, John M Mitchell
Rok vydání:	1999
Předmět:	Pharmacology Residue (complex analysis) Bovine milk Chromatography Chemistry Sulfadimidine Contamination Antimicrobial Analytical Chemistry medicine Environmental Chemistry Test performance Test protocol Agronomy and Crop Science Food Science medicine.drug Antibacterial agent
Zdroj:	Journal of AOAC INTERNATIONAL. 82:79-84
ISSN:	1944-7922 1060-3271
DOI:	10.1093/jaoac/82.1.79
Popis:	LacTekTM tests are competitive enzyme-linked immunosorbent assays intended for rapid detection of antimicrobial residues in bovine milk. In this study, the LacTek test protocol was modified for use with extracts of bovine tissue to detect β-lac-tam, tetracycline, and sulfamethazine residues. Testperformance characteristics—precision, accuracy, ruggedness, practicability, and analytical specificity and sensitivity—were investigated. Results suggest that LacTek tests can be easily adapted to detect antimicrobial residues in extracts of lean ground beef. However, positive samples may not contain residues at violative concentrations (i.e., Canadian maximum residue limits), and therefore, additional analysis would be required for final confirmation and quantitation (e.g., chromatography).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::30bdb79f4ca63b4d9d110f14687f7077 https://doi.org/10.1093/jaoac/82.1.79 Zobrazit plný text záznamu