Abstract P249: ICV Infusion Of AgRP Modulates Feeding Behavior And Energy Expenditure In Mice

Autor:	Curt D. Sigmund, Kirthikaa Balapattabi, Sebastiao Donato Silva, Justin L. Grobe, Vanessa Oliveira, Chetan N Patil, John J Reho
Rok vydání:	2020
Předmět:	medicine.medical_specialty digestive oral and skin physiology Metabolism Biology Feeding behavior Endocrinology Energy expenditure Arcuate nucleus Internal medicine Renin–angiotensin system Internal Medicine medicine Genetic ablation Receptor Gene
Zdroj:	Hypertension. 76
ISSN:	1524-4563 0194-911X
DOI:	10.1161/hyp.76.suppl_1.p249
Popis:	A subset of Agouti-related protein (AgRP) neurons within the arcuate nucleus express angiotensin type 1a receptors (AT1A), and genetic ablation of AT1A in these cells disinhibits AgRP gene expression and attenuates energy expenditure (EE) in mice. To further understand the role of AgRP in metabolic control, and to establish relevant dosing schedules in this species, here we tested the effect of intracerebroventricular infusion (icv) of recombinant AgRP on metabolic functions in C57BL/6J male mice. First, we examined the effects of AgRP(82-132) (21 days, 1 or 10 nmol/d, icv) or artificial cerebrospinal fluid (aCSF) using metabolic cages. High dose AgRP reduced body mass (aCSF n=12, +0.8±0.6 vs 1 nmol/d n=14, +0.6±0.6 vs 10 nmol/d n=11, -1.5±0.5 p
Databáze:	OpenAIRE
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