Cloning of a DNA fragment involved in pigment production in Streptomyces avermitilis

Autor:	Claudio D. Denoya, M R Morgenstern, Deborah D. Skinner, M.W. Tibbetts, E.W. Hafner
Rok vydání:	1992
Předmět:	Genetics Cloning Streptomycetaceae lac operon Molecular cloning Biology biology.organism_classification medicine.disease_cause Microbiology Streptomyces Biochemistry medicine sense organs Molecular Biology Escherichia coli Gene Streptomyces avermitilis
Zdroj:	FEMS Microbiology Letters. 91:9-13
ISSN:	1574-6968 0378-1097
DOI:	10.1111/j.1574-6968.1992.tb05176.x
Popis:	Streptomyces avermitilis has the ability to synthesize a diffusible, brown, melanin-like pigment, a common property among many Streptomyces species. A region of the S. avermitilis chromosome involved in the production of this pigment was cloned in Escherichia coli. Production of the brown pigment was attained in E. coli, and is optimal when medium is supplemented with copper ions, tyrosine and IPTG. The cloned S. avermitilis pigment-producing DNA fragment is under the control of the lac promoter carried in the E. coli vector. The gene involved in pigment production could be used as a tool to analyse gene expression in S. avermitilis, and as an alternative cloning marker in Streptomyces-Escherichia coli vectors.
Databáze:	OpenAIRE
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