Synthesis of artificial antigens of diethylstilbestrol and preparation of its antibody
| Autor: | Xianghong Wang, Qiujia Fan, Qunbiao Zhou, Yuan Zhao, Li Wang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
endocrine system
biology medicine.diagnostic_test Chemistry Diethylstilbestrol Molecular biology Ovalbumin Immune system Biochemistry Antigen Western blot biology.protein medicine Antibody Bovine serum albumin Agronomy and Crop Science Hapten hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Frontiers of Agriculture in China. 4:188-194 |
| ISSN: | 1673-744X 1673-7334 |
| DOI: | 10.1007/s11703-010-0097-8 |
| Popis: | Diethylstilbestrol-hemi-succinate (DES-HS) and diethylstilbestrol-monocarboxylic-propyl-ether (DESMCPE) were synthesized in different ways. The two kinds of haptens were conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to form the immuno-antigens and the coating-antigen, respectively. The antibody with high titer was obtained after immunising rabbits. The results showed that the antibody had a high affinity to diethylstilbestrol tested by enzyme-linked immunosorbent assay (ELISA) and Western blot. This study lays a basis for further research on the immune test kit for the DES remnant. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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