Comparative analysis of free-living and particle-associated bacterial communities of Lake Baikal during the ice-covered period
| Autor: | Igor V. Khanaev, Yulia R. Zakharova, Darya P. Petrova, Maria V. Bashenkhaeva, Maria V. Sakirko, Yelena V. Likhoshway, Yuri P. Galachyants |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Cyanobacteria Operational taxonomic unit Ecology biology 010604 marine biology & hydrobiology Verrucomicrobia 010501 environmental sciences Aquatic Science Plankton biology.organism_classification 01 natural sciences Haptophyte Diatom Water column Dinobryon Botany Environmental science Ecology Evolution Behavior and Systematics 0105 earth and related environmental sciences |
| Zdroj: | Journal of Great Lakes Research. 46:508-518 |
| ISSN: | 0380-1330 |
| DOI: | 10.1016/j.jglr.2020.03.015 |
| Popis: | Free-living (FLC) and particle-associated (PAC) bacterial communities contribute differentially to the cycling of matter in the environment. In this work we have for the first time studied the taxonomic composition and diversity of FLC and PAC in the oligotrophic freshwater Lake Baikal during the under-ice plankton peak. The communities on the bottom ice surface and in the water column under the ice were studied. Analysis by light microscopy showed that the bottom surface of the ice was dominated by green algae Chlorella spp. A complex of microalgae developed in the water column: green algae Monoraphidium griffithii, Monoraphidium contortum, Chlorella spp., chrysophyte Dinobryon cylindricum, haptophyte Chrysochromulina parva, and diatom Synedra acus. Algal abundance on the ice sub-surface was several orders of magnitude higher than that in the water column. According to pyrosequencing data of the bacterial 16S rRNA V3-V4 region, diversity of FLC (as measured by Shannon, Simpson, Chao1 and ACE indices) was higher than that of PAC. Proteobacteria (Burkholderiaceae) dominated the FLC associated with ice; FLC of the water column under ice were dominated by Actinobacteria (Sporichthyaceae and Ilumatobacteraceae) and Verrucomicrobia (Methylacidiphilaceae). PAC associated with ice were dominated by Bacteroidetes (Flavobacteriaceae), while water column-associated PAC were dominated by Verrucomicrobia (FukuN18_freshwater_group, Methylacidiphilaceae) and cyanobacteria (Cyanobiaceae). The difference between free-living and particle-associated, as well as between water column and ice sub-surface bacterial communities, were explored on operational taxonomic unit (OTU) and phylotype levels. |
| Databáze: | OpenAIRE |
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