Development of Methods for the Target-Specific Protein Elimination in Plants

Autor:	I A Abdeeva, S. A. Bruskin, J. S. Panina, L. G. Maloshenok, A. D. Zolotarenko, E. S. Piruzian
Rok vydání:	2018
Předmět:	0301 basic medicine chemistry.chemical_classification fungi Biology Yeast Amino acid Ubiquitin ligase Green fluorescent protein 03 medical and health sciences 030104 developmental biology chemistry Ubiquitin Proteasome Biochemistry Genetics biology.protein Fluorescence microscope Gene
Zdroj:	Russian Journal of Genetics. 54:1378-1382
ISSN:	1608-3369 1022-7954
Popis:	Here we present the method for the target-specific elimination of certain intracellular proteins in plants using the ubiquitin-proteasome system. We modified the E3 ubiquitin ligase Chip of A. thaliana to obtain two variants carrying the deletions at the N-terminus and the GFP recognition domain. The interaction of the GFP protein and the chimeric ubiquitin ligase was confirmed via yeast two-hybrid assay. Fluorescence microscopy and fluorimetry showed that, when an infiltration of the gfp-expressing N. benthamiana plants was performed with agrobacteria carrying the hybrid E3 gene of the ubiquitin ligase Chip with the GFP recognition domain, a significant decrease in the fluorescence was observed for both variants: carrying the N-terminal deletion of 100 amino acids or the deletion of 140 amino acids.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2ebf47adac3b2f1d0c6e6515909c9663 https://doi.org/10.1134/s1022795418110091 Zobrazit plný text záznamu Full text from SpringerLink