Effects of sulfur and monensin concentrations on in vitro dry matter disappearance, hydrogen sulfide production, and volatile fatty acid concentrations in batch culture ruminal fermentations1

Autor:	J. Leibovich, Nicolas DiLorenzo, M. J. Quinn, M.L. May, D.R. Smith, J. W. Homm, M. L. Galyean
Rok vydání:	2010
Předmět:	chemistry.chemical_classification Monensin General Medicine Distillers grains chemistry.chemical_compound Animal science Biochemistry chemistry Genetics Urea Hay Propionate Animal Science and Zoology Dry matter Fermentation Cottonseed meal Food Science
Zdroj:	Journal of Animal Science. 88:1503-1512
ISSN:	1525-3163 0021-8812
DOI:	10.2527/jas.2009-2498
Popis:	Effects of monensin (MON) and S on in vitro fermentation and H(2)S production were evaluated in 2 experiments. In Exp. 1, 2 ruminally cannulated steers were adapted (>14 d) to a 75% concentrate diet [steam-flaked corn (SFC)-based], and ruminal fluid was collected approximately 4 h after feeding. Substrate composed (DM basis) of 85.2% SFC, 9% alfalfa hay, 5% cottonseed meal, and 0.8% urea was added with ruminal fluid and buffer to sealed 125-mL serum bottles to allow for gas collection. A Na(2)SO(4) solution was added to yield S equivalent to 0.2, 0.4, and 0.8% of substrate DM, and MON was included at 0, 2, 4, and 6 mg/L of culture volume. Bottle head-space gas was analyzed for H(2)S. No MON (P = 0.29) or MON x S interaction (P = 0.41) effects were detected for H(2)S production. Increasing S linearly increased (P 21 d) to a 75% concentrate diet (SFC base) that contained 15% (DM basis) wet corn distillers grains plus solubles (WDGS) and MON at 22 mg/kg of DM. In vitro substrate DM was composed of 75.4% SFC, 15% WDGS, 9% alfalfa hay, and 0.6% urea, and S and MON concentrations were the same as in Exp. 1. No effects of MON (P = 0.93) or the MON x S interaction (P = 0.99) were noted for H(2)S production; however, increasing S linearly increased (P < 0.01) H(2)S production. No effects of MON (P = 0.16), S (P = 0.43), or the MON x S interaction (P = 0.10) were noted for IVDMD (average 70.9%). Total VFA concentrations were not affected by MON (P = 0.40), S (P = 0.26), or the MON x S interaction (P = 0.59). As in Exp. 1, increasing MON linearly decreased (P < 0.05) molar proportions of acetate, butyrate, and A:P and linearly increased (P < 0.01) propionate. Increasing S concentration increased in vitro H(2)S production, but S did not affect VFA concentrations and proportions. Monensin did not affect in vitro H(2)S production, and changes in VFA molar proportions were evident with MON regardless of S concentration.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2e644a8c8875bf4cdbdc30de8b546396 https://doi.org/10.2527/jas.2009-2498 Zobrazit plný text záznamu