The myrobalan (Prunus cerasifera L.): a useful diploid model for studying the molecular genetics of self-incompatibility in plums

Autor: T. P. Robbins, Kenneth R. Tobutt, B. G. Sutherland, R. Cerovič
Rok vydání: 2008
Předmět:
Zdroj: Euphytica. 166:385-398
ISSN: 1573-5060
0014-2336
DOI: 10.1007/s10681-008-9821-3
Popis: A series of PCR methods were used to detect S-RNase alleles and SFB alleles and to determine S-genotypes in 25 accessions of myrobalan (Prunus cerasifera L.). Firstly, primers flanking the polymorphic second intron were used to identify S-RNases in agarose gels. These primers amplified one or two bands per accession in 25 accessions. Then consensus primers were designed for amplifying the polymorphic first intron, unique to Prunus S-RNases, for automated fluorescent detection. Each accession produced one or two peaks. New primers were then developed to amplify the intron in the SFB gene, for detection by fluorescence. Cross-referencing PCR bands and peaks indicated 15 S-alleles were present in the 25 accessions. Cloning, sequencing and comparison with published data indicated that the amplified products were S-RNase alleles. Sequence information was used to design primers specific for each S-RNase. Full and consistent S-genotypes were obtained by cross-comparing PCR data for 23 of the 25 accessions, and two accessions appeared to have a single allele. Pollen-tube microscopy indicated function of some but not all of the S-alleles sequenced.
Databáze: OpenAIRE
Externí odkaz: