SIMULTANEOUS DETERMINATION OF GLIMEPIRIDE AND ATORVASTATIN IN HUMAN SERUM BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY: APPLICATION TO PHARMACOKINETIC STUDY

Autor: Harshini Kotagiri, Chinna Reddy Palem, Madhsusudan Rao Yamsani, Ramesh Gannu, Vamshi Vishnu Yamsani, Shravan Kumar Yamsani
Rok vydání: 2011
Předmět:
Zdroj: Journal of Liquid Chromatography & Related Technologies. 34:2420-2432
ISSN: 1520-572X
1082-6076
DOI: 10.1080/10826076.2011.591023
Popis: A simple and sensitive high performance liquid chromatographic method for simultaneous quantification of glimepiride (GMP) and atorvastatin (ATN) in human serum was developed and validated. GMP, ATN, and internal standard (IS), pioglitazone (PG) were extracted into dichloromethane and methanol solvent system and separated using an isocratic mobile phase on an Inertsil C18 column. The eluent was monitored by UV detector at 230 nm. The linearity range of proposed method was 1–1600 ng mL−1 for each analyte. The retention times for GMP, ATN, and IS were found to be 9.8, 6.92, and 7.96 min, respectively. The intra-day and inter-day coefficient of variation and percentage error values of the assay method were less than 15% and mean recovery was more than 96, 93, and 95% for GMP, ATN, and IS, respectively, and the method was found to be precise, accurate, and specific. The method was successfully applied for pharmacokinetic study of GMP and ATN after oral administration to patients. The CMax, TMax, and AUC0–12 o...
Databáze: OpenAIRE
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