Carbon-dot–hydrogel for enzyme-mediated bacterial detection
| Autor: | Sukhendu Nandi, Sagarika Bhattacharya, Raz Jelinek |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
Quenching (fluorescence) biology General Chemical Engineering technology industry and agriculture macromolecular substances 02 engineering and technology General Chemistry Matrix (biology) 010402 general chemistry 021001 nanoscience & nanotechnology Cleavage (embryo) biology.organism_classification complex mixtures 01 natural sciences Esterase Fluorescence 0104 chemical sciences Transduction (biophysics) Enzyme chemistry Biochemistry Biophysics 0210 nano-technology Bacteria |
| Zdroj: | RSC Advances. 7:588-594 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c6ra25148j |
| Popis: | A hybrid carbon-dot (C-dot)–hydrogel matrix was constructed and employed for detection of bacteria. The transduction mechanism is novel, based upon cleavage of ester bonds within the hydrogel scaffold by bacterially-secreted esterases; the ensuing fluidization of the hydrogel resulted in aggregation of the embedded C-dots and consequent quenching of their fluorescence. We show that the C-dot–hydrogel exhibits high sensitivity and can distinguish among bacterial species through modulation of the emitted fluorescence, depending upon their esterase secretions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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