Biliary excretion of taurocholate, organic anions and vinblastine in rats with α-naphthylisothiocyanate-induced cholestasis

Autor: Hiroko Kurihara, Hajime Takikawa, Naoyo Sano
Rok vydání: 2005
Předmět:
Zdroj: Journal of Gastroenterology and Hepatology. 20:1069-1074
ISSN: 0815-9319
Popis: Background and Aims: α-Naphthylisothiocyanate (ANIT) is known to cause cholestasis due to injury of the bile duct epithelial cells. The aim of the present study was to examine the effect of a single dose of ANIT on the biliary excretion of various cholephilic compounds and on the amount of canalicular transporters. Methods: Twenty-four hours after the oral administration of ANIT (100 mg/kg), the biliary excretion of taurocholate, leukotriene C4, pravastatin and vinblastine was studied. The protein levels of the bile salt export pump and multidrug resistance protein 2 and the immunostaining of multidrug resistance protein 2 in the liver were also examined. Results: The ANIT treatment markedly decreased the biliary excretion of tracer amounts of taurocholate, leukotriene C4, pravastatin and vinblastine. The biliary excretory maximum of taurocholate was also markedly decreased after ANIT treatment. The ANIT treatment had no effect on the protein levels of bile salt export pump and multidrug resistance protein 2 and the immunostaining of multidrug resistance protein 2 in the liver. Conclusions: These findings support canalicular transporters having little effect on the marked impairment of biliary excretion of cholephilic compounds in ANIT-induced cholestasis. © 2005 Blackwell Publishing Asia Pty Ltd
Databáze: OpenAIRE
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