The Effect of Melanocyte Stimulating Hormone and Hydroxyapatite on Osteogenesis in Pulp Stem Cells of Human Teeth Transferred into Polyester Scaffolds
| Autor: | Effat Alizadeh, Alexander M. Seifalian, Neda Jabbari, Roya Salehi, Mohammad Samiei, Vahideh Raeisdasteh Hokmabad, Marziyeh Aghazadeh |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science Polymers and Plastics General Chemical Engineering Osteoblast 02 engineering and technology General Chemistry 021001 nanoscience & nanotechnology Cell biology 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Tissue engineering Nanofiber Dental pulp stem cells medicine Alkaline phosphatase Pulp (tooth) MTT assay Viability assay 0210 nano-technology |
| Zdroj: | Fibers and Polymers. 19:2245-2253 |
| ISSN: | 1875-0052 1229-9197 |
| DOI: | 10.1007/s12221-018-8309-6 |
| Popis: | Presently, tissue engineering is employed in the restoration and repair of tissue defects. Degradable scaffolds, stem cells and stimulating factors are employed in this method. In this study, the effect of melanocyte-stimulating hormone (MSH) and/or hydroxyapatite (HA) on proliferation, osteoblast differentiation, and mineralization of human dental pulp stem cells (hDPSCs) seeded on PLLA-PCL nanofibrous scaffolds was evaluated. For this aim, PLLA-PCL-HA nanofibrous scaffolds were fabricated using electrospinning method. FE-SEM images exhibited that all nanofibers had bead-free morphologies with average diameters ranging from 150–205 nm. Human DPSCs seeded into PLLA-PCL nanofibers were treated with MSH. Cell viability, proliferation, morphology, osteogenic potential, and the expression of tissue-specific genes were assessed by means of MTT assay, FE-SEM, alizarin red S staining, and RT-PCR analysis. hDPSCs exhibited improved adhesion and proliferation capacity on the PLLA-PCL-HA nanofibers treated with MSH compared to other groups (p |
| Databáze: | OpenAIRE |
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