Imposed starvation forin vitromaintenance and high regeneration of elite key lime (Citrus aurantifolia)
| Autor: | Mohammad Reza Safarnejad, Naser Farrokhi, S. Soheilivand, Amir Mousavi, Mohsen Mardi, Masoud Tohidfar, Seyed Mehdi Alavi |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Starvation Regeneration (biology) food and beverages Horticulture Biology engineering.material 01 natural sciences In vitro 03 medical and health sciences 030104 developmental biology Micropropagation Shoot Genetics engineering medicine medicine.symptom 010606 plant biology & botany Lime Explant culture Plant stem |
| Zdroj: | The Journal of Horticultural Science and Biotechnology. 93:474-482 |
| ISSN: | 2380-4084 1462-0316 |
| DOI: | 10.1080/14620316.2017.1415702 |
| Popis: | Preservation of elite key lime (Citrus aurantifolia) genotypes using efficient micropropagation and regeneration protocols is very important for new breeding programmes. The objective of the present study was to develop an efficient protocol for micropropagation and maintenance of elite genotypes of key lime based on slow growth and starvation strategy. To do this, regenerated shoots were starved, and the growth was compromised via avoiding subcultures for 4, 8, 12 and 24 months. The numbers of responsive explants and adventitious shoots per each explant were evaluated in the presence of benzylaminopurine (BAP). The results showed that the 12- and 24-month starved explants treated with 1 mg/l BAP were able to produce four times more shoots than the control. In addition, the influences of horizontal or vertical layering explants over medium were evaluated. The results indicated that all horizontally layered explants significantly produced 25% adventitious shoots more than vertical explants, most li... |
| Databáze: | OpenAIRE |
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