Abstract P233: Microvascular Fingerprint Of Multi-Organ Imaging - New Insights In Preeclampsia Research
| Autor: | Hanna Napieczynska, Nadine Haase, Kristin Kraeker, Ralf Dechend, Arnd Heuser, Dominik N. Müller, Sarah Kedziora |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Hypertension. 78 |
| ISSN: | 1524-4563 0194-911X |
| DOI: | 10.1161/hyp.78.suppl_1.p233 |
| Popis: | To establish the first protocol for high-resolution three-dimensional imaging of multiple organs in rodents, we performed microvasculature casting with Microfil silicone rubber substance in non-living rats and combined the method with advanced image analysis protocols after micro-CT scanning. This newly established method allows changes in individual organs to be visualized and examined in extraordinary detail as part of a multi-organ approach. Previously published protocols only address visualization of single organs or are insufficiently resolved with respect to vessel diameter analysis. With optimized staining protocols, samples can additionally be examined for possible structural changes such as inflammatory processes or fibrotic formation. Preeclampsia is a condition during pregnancy that leads to up to a 4-fold increased risk of maternal cardiovascular disease later in life, although symptoms usually resolve after delivery. In former preeclamptic rats, a reduction in microvascular density in the heart and brain with a simultaneous increase in retinal vessels has been described, even weeks after the disappearance of major features such as hypertension and albuminuria. The technique described will enhance studies of the vasculature in many other animal models and provide striking insights into vascular physiology necessary to drive translation. |
| Databáze: | OpenAIRE |
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