Expression of 60-kDa and inducible 70-kDa stress proteins in gentamicin-induced acute renal failure

Autor: Yohtalou Tashima, Atsushi Komatsuda, Guo-Zhong Tao, Hideaki Itoh, Akira B. Miura
Rok vydání: 1997
Předmět:
Zdroj: Clinical and Experimental Nephrology. 1:254-260
ISSN: 1437-7799
1342-1751
DOI: 10.1007/bf02480637
Popis: Heat shock proteins contribute to the survival of cells under stress conditions. We have previously observed the expression of HSP90 and constitutive HSP70 in rat kidneys during stress loading. This study was designed to determine whether HSP60 and HSP70i are induced in rat kidneys during exposure to gentamicin. During the continuous administration of gentamicin (40 mg/kg per day) to rats for 30 days, the changes of HSP60 and HSP70i in these kidneys were quantitatively investigated by immunoblot analyses using specific antibodies. The indicators for renal failure,N-acetyl-β-d-glucosaminidase, blood urea nitrogen, and serum creatinine, increased, and reached the maximum levels at day 12. Thereafter, they spontaneously decreased, and were close to their normal levels at day 21, despite continued gentamicin exposure. The time course of HSP60 levels showed a unique 2-peak pattern exclusively in the cytosols of the cortex; the gentamicin target site. The second HSP60 peak occurred during the recovery phase, and coincided with published reports of a second peak of gentamicin accumulation in the kidney. The time course of HSP70i level showed a single peak at day 12 when the renal failure was most intense; the level was similar between the soluble and insoluble fractions in all portions of the kidney, except the inner medulla. The increase in cytosolic HSP60 levels, as well as HSP70i levels, may be related to the spontaneous recovery from renal failure at the target site of the drug.
Databáze: OpenAIRE
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