Cloning and expression of BTB/POZ-domain from zebrafish gcl (germ cell-less), and appraisement of its polyclonal-antibody

Autor:	Qiang Lan, Fengjiao Deng, Tiangang Zhuang, Yan Xu, Peng-Xiang Cheng
Rok vydání:	2008
Předmět:	Genetics Cloning Multidisciplinary Expression vector biology medicine.diagnostic_test Chemistry biology.organism_classification Fusion protein Cell biology law.invention Western blot law Polyclonal antibodies Recombinant DNA medicine biology.protein BTB/POZ domain Zebrafish
Zdroj:	Wuhan University Journal of Natural Sciences. 13:123-128
ISSN:	1993-4998 1007-1202
DOI:	10.1007/s11859-008-0124-2
Popis:	In this study, a recombinant pET28c-gBTB/POZ was constructed by cloning the sequence of the BTB/POZ domain of the zebrafish gcl (germ cell-less) into the expression vector pET28c, and pET28c-gBTB/POZ was transformed into BL21(DE3) pLysS strain to express the fusion protein for the preparation of antibody. Polyclonal-antibody against the GCL-BTB/POZ domain was prepared by immunizing rabbit with the fusion protein, and the Western Blot and immuno-histochemical analysis were performed to detect the quantity of the polyclonal-antibody. The result indicates that the polyclonal-antibodies were of good quantity and specification. Further studies will be performed to demonstrate the function and expression pattern of the GCL protein during the development process of zebrafish with the polyclonal-antibody.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2aaf9af822855e337b1c1a121ccafb77 https://doi.org/10.1007/s11859-008-0124-2 Zobrazit plný text záznamu Full text from SpringerLink