Use of High-Performance Liquid Chromatography–Mass Spectrometry of Adipose Tissue for Detection of Bioaccumulation of Pyriproxyfen in Adults of Lithobates catesbeianus
| Autor: | Vladimir Pavan Margarido, Natalicio Ferreira Leite, Rafaela Maria Moresco, Jardel Nimet, Alex Fabiano Paulin |
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| Rok vydání: | 2021 |
| Předmět: |
Amphibian
Veterinary medicine biology Health Toxicology and Mutagenesis Lithobates Adipose tissue General Medicine Pesticide Toxicology biology.organism_classification Pollution High-performance liquid chromatography chemistry.chemical_compound chemistry Bioaccumulation biology.animal Ecotoxicology Pyriproxyfen |
| Zdroj: | Bulletin of Environmental Contamination and Toxicology. 107:911-916 |
| ISSN: | 1432-0800 0007-4861 |
| DOI: | 10.1007/s00128-021-03356-8 |
| Popis: | The aims of the study were: (i) evaluate the efficacy of using amphibian adipose tissue as a valid biomarker of water contaminated by pyriproxyfen; and (ii) verify the use of Lithobates catesbeianus in laboratory experiments as a potential bioindicator for bioaccumulation of pesticide in adipose tissue from amphibians. Adult frogs were exposed to different dilutions (0.002 g/L and 0.02 g/L) of Sumilarv® (pyriproxyfen) over 50 days. The average results of the fortified sample were 108%, indicating that the test method was effective. Adult frogs exposed to the standard dose recommended by the World Health Organization (WHO) showed bioaccumulation of pyriproxyfen in adipose tissue significantly higher than control animals. Lithobates catesbeianus proved to be an effective bioindicator and the adipose tissue was an efficient biomarker to bioaccumulation of pyriproxyfen. We conclude that high-performance liquid chromatography–mass spectrometry was effective measuring pyriproxyfen bioaccumulation in adult amphibians. |
| Databáze: | OpenAIRE |
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