Decreased wild-type full-length Et-A and -B receptors in neuroblastoma and Ewing sarcoma cells
| Autor: | Y.F. Zhang, N.D. Carter, P.A. Berry, Susan A. Burchill, S. Jeffery |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.hormone
Cancer Research medicine.medical_specialty Chinese hamster ovary cell Neural crest Transfection Biology medicine.disease Molecular biology Endothelins Endocrinology Oncology Cell culture Neuroblastoma Internal medicine Pediatrics Perinatology and Child Health medicine Endothelin receptor Receptor |
| Zdroj: | Medical and Pediatric Oncology. 36:142-146 |
| ISSN: | 1096-911X 0098-1532 |
| DOI: | 10.1002/1096-911x(20010101)36:1<142::aid-mpo1034>3.0.co;2-z |
| Popis: | Background Endothelins and their receptors, Et-A and Et-B, play an essential role in differentiation and migration of neural crest cells. Expression of endothelin receptors has been examined in neuroblastoma and Ewing sarcoma cell lines. Procedure RNA was amplified for Et-A and Et-B by RT-PCR. Amplified products were cloned into the expression vector pLNCX, which was used to transfect CHO cells. Binding characteristics of transfected CHO cells were examined. Results Full-length Et-A mRNA was identified in all cell lines, in addition to a truncated Et-A product. CHO cells expressing full-length Et-A bound to endothelin, but cells expressing truncated Et-A did not. Full length Et-B mRNA was not detected, but two smaller molecular weight products were amplified. These are as yet uncharacterised. Conclusions These results suggest that endothelins and their receptors may be important in the development and biology of neuroblastoma and Ewing sarcoma. Med. Pediatr. Oncol. 36:142–146, 2001. © 2001 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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