Production of a herbicide, 5-aminolevulinic acid, by Rhodobacter sphaeroides using the effluent of swine waste from an anaerobic digestor
| Autor: | Ken Sasaki, Mitsunori Hayashi, Yoshinori Nishizawa, Tohru Tanaka |
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| Rok vydání: | 1990 |
| Předmět: |
biology
Chemistry Porphobilinogen synthase General Medicine biology.organism_classification Applied Microbiology and Biotechnology Rhodobacter sphaeroides chemistry.chemical_compound Biochemistry Biosynthesis Glycine biology.protein Levulinic acid Rhodospirillales Rhodospirillaceae Bacteria Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 32:727-731 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | For the production of a herbicide, 5-amino-levulinic acid (ALA), from anaerobic digestion liquor, the utilization of the photosynthetic bacterium, Rhodobacter sphaeroides was examined. This bacterium could produce ALA extracelularly from this liquor with the addition of levulinic acid (LA), an inhibitor of ALA dehydratase (ALAD), and glycine, a precursor of ALA biosynthesis in the Shemin pathway. Succinate (another precursor) addition was unnecessary for ALA production. When repeated additions of LA were made together with glycine ALA production was significantly enhanced. However, above three additions of LA, ALA production was not further enhanced. The maximum value of ALA production attained was 4.2 mM (0.63 g/ 1), which was over double that of other ALA producers such as Chlorella vulgaris. Propionic acid was predominantly utilized compared with other lower fatty acids, suggesting that this might be converted to ALA via succinyl-coenzyme A (CoA) in the methylmalonyl-CoA pathway. |
| Databáze: | OpenAIRE |
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