Fractional laser-assisted drug delivery: Laser channel depth influences biodistribution and skin deposition of methotrexate
| Autor: | Catharina M. Lerche, Merete Haedersdal, R. Rox Anderson, E.H. Taudorf, Steen Honoré Hansen, Uwe Paasch, Christian Janfelt, Peter A. Philipsen, Andrés M. Erlendsson |
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| Rok vydání: | 2016 |
| Předmět: |
Biodistribution
Pathology medicine.medical_specialty Chemistry medicine.medical_treatment Dermatology Ablation 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure 030220 oncology & carcinogenesis Drug delivery medicine Distribution (pharmacology) Surgery Methotrexate Epidermis Deposition (chemistry) Er:YAG laser medicine.drug Biomedical engineering |
| Zdroj: | Lasers in Surgery and Medicine. 48:519-529 |
| ISSN: | 0196-8092 |
| DOI: | 10.1002/lsm.22484 |
| Popis: | Background and Objective Ablative fractional laser (AFXL) facilitates delivery of topical methotrexate (MTX). This study investigates impact of laser-channel depth on topical MTX-delivery. Materials and Methods MTX (1% [w/v]) diffused for 21 hours through AFXL-exposed porcine skin in in vitro Franz Cells (n = 120). A 2,940 nm AFXL generated microscopic ablation zones (MAZs) into epidermis (11 mJ/channel, MAZ-E), superficial-dermis (26 mJ/channel, MAZ-DS), and mid-dermis (256 mJ/channel, MAZ-DM). High performance liquid chromatography (HPLC) was used to quantify MTX deposition in full-thickness skin, biodistribution profiles at specific skin levels, and transdermal permeation. Fluorescence microscopy was used to visualize UVC-activated MTX-fluorescence (254 nm) and semi-quantify MTX distribution in skin. Results AFXL increased topical MTX-delivery (P |
| Databáze: | OpenAIRE |
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