Preparation of Decellularized Biological Scaffolds for 3D Cell Culture
| Autor: | Bryan N. Brown, Michael J. Buckenmeyer, Travis A. Prest |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Decellularization Chemistry 02 engineering and technology Matrix (biology) 021001 nanoscience & nanotechnology Cell biology Extracellular matrix 03 medical and health sciences 3D cell culture 030104 developmental biology medicine.anatomical_structure Dermis Cell culture Self-healing hydrogels medicine 0210 nano-technology C2C12 |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493970193 |
| DOI: | 10.1007/978-1-4939-7021-6_2 |
| Popis: | The biggest challenge of designing and implementing an in vitro study is developing a microenvironment that most closely represents the interactions observed in vivo. Decellularization of tissues and organs has been shown to be an effective method for the removal of potentially immunogenic constituents while preserving essential growth factors and extracellular matrix (ECM ) proteins necessary for proper cell function. Enzymatic digestion of decellularized tissues allows these tissue-specific components to be reconstituted into bioactive hydrogels through a physical crosslinking of collagen. In the following protocol, we describe unique decellularization methods for both dermis and urinary bladder matrix (UBM) derived from porcine tissues. We then provide details for hydrogel formation and subsequent three-dimensional (3D) culture of two cell types: NIH 3T3 fibroblasts and C2C12 myoblasts . |
| Databáze: | OpenAIRE |
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