Complexes of telomeric oligonucleotides with the PGEk protein vector: Internalization by target cells and antiproliferative activity

Autor:	Galina E. Pozmogova, A. N. Chuvilin, E. S. Severin, N. N. Kireeva, G. A. Posypanova
Rok vydání:	2008
Předmět:	Telomerase Nuclease Oligonucleotide Cell growth media_common.quotation_subject Biophysics Biology law.invention Biochemistry Structural Biology Epidermal growth factor law Recombinant DNA biology.protein Internalization Receptor media_common
Zdroj:	Molecular Biology. 42:253-260
ISSN:	1608-3245 0026-8933
DOI:	10.1134/s0026893308020118
Popis:	Recombinant protein PGEk, consisting of the human epidermal growth factor (hEGF) and a DNA-binding domain, proved to be capable of interacting with the hEGF receptor and inducing cell proliferation, as characteristic of hEGF. PGEk complexes with the telomere-mimic oligonucleotide d(TTAGGG)4 (TMO) and its thio analog (TMS) were efficiently and selectively internalized by cells with a high-level expression of the hEGF receptors. The extent of internalization was studied as a function of the PGEk: oligonucleotide ratio in the complex. The intracellular location of the oligonucleotides was determined. PGEk was found to ensure a more efficient delivery of the oligonucleotides and to protect them from nuclease degradation. The oligonucleotides contained in the complexes exerted a far greater cytotoxic effect as compared with the free oligonucleotides.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::28ac45dff6522213416413364cbb97ef https://doi.org/10.1134/s0026893308020118 Zobrazit plný text záznamu Full text from SpringerLink