Primary mouse brain pericytes isolated from transgenic Alzheimer mice spontaneously differentiate into a CD11b+ microglial-like cell type in vitro
| Autor: | Bianca Hutter-Schmid, Christian Humpel |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Aging Cell type Microglia biology Amyloid beta Transgene Cell Biology Biochemistry In vitro Cell biology Fibronectin 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Endocrinology medicine.anatomical_structure Integrin alpha M Genetics medicine biology.protein Pericyte Molecular Biology 030217 neurology & neurosurgery |
| Zdroj: | Experimental Gerontology. 112:30-37 |
| ISSN: | 0531-5565 |
| DOI: | 10.1016/j.exger.2018.08.003 |
| Popis: | Alzheimer's disease (AD) is characterized by amyloid-β plaques, tau pathology and vascular impairment including pericyte damage. Pericytes are perivascular cells of the blood-brain barrier and can differentiate into different cell types in vitro including microglia. The aim of the present study is to explore if primary mouse brain pericytes isolated and cultured from transgenic AD (APP_SweDI) mice can differentiate into CD11b+ (integrin alpha M) microglia in vitro. We show that primary pericytes (passage 5) isolated from wildtype C57BL6 mice differentiated into CD11b+ microglia (Type B, >90%), when exposed to a differentiation factor mix of FGF-2, cAMP and fibronectin. This differentiation was time-dependent and seen as a large 80 kDa CD11b fragment (days 1–8) and a smaller 50 kDA CD11b fragment (>4 days). These pericytes did not differentiate into neurons, astroglia or oligodendroglia. However, pericytes isolated from transgenic AD mice differentiated into CD11b+ microglia (Type A |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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