Expression and regulation of the yeastMEL1gene
| Autor: | Pirjo Lehtinen, Helena T. Kopu, Nisse Kalkkinen, Matti Korhola, Pirkko L. Liljestrom, Tuula K. Torkkeli, Hannele Ruohola |
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| Rok vydání: | 1986 |
| Předmět: |
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0303 health sciences biology 030306 microbiology Saccharomyces cerevisiae Periplasmic space biology.organism_classification Microbiology Molecular biology Endoglycosidase 03 medical and health sciences Biochemistry Transcription (biology) Gene expression Genetics Extracellular biology.protein Molecular Biology Gene 030304 developmental biology |
| Zdroj: | FEMS Microbiology Letters. 34:179-185 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.1986.tb01400.x |
| Popis: | The Saccharomyces cerevisiae var. uvarum MEL1 gene, conferring production of α-galactosidase, has been cloned to study its expression. Increasing amounts of α-galactosidase protein and activity closely followed the induction of functional MEL1 transcripts (1.65 kb). The initial appearance of the transcript occurred within 3–6 min and the corresponding α-galactosidase activity within 8–10 min of galactose addition. Therefore, regulation of α-galactosidase synthesis operates primarily at the level of MEL1 transcription. No transcription is detectable in glucose-grown cultures. α-Galactosidase activity is found in the periplasmic space and in the extracellular medium. Enzyme from either location exhibited a molecular size of 76 kDa, which was reduced to 53 kDa after deglycosylation with endoglycosidase H. The N-terminal sequence of purified α-galactosidase reveals cleavage of putative 18-amino-acid signal sequence for secretion. |
| Databáze: | OpenAIRE |
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