Development and Validation of a HPLC Method for the Simultaneous Determination of Ketoprofen Lysine Salt and Preservative in OKI® Spray
| Autor: | Giuseppe Carlucci, Paolo Vigilante, Roberto Anacardio |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Ketoprofen
chemistry.chemical_classification Preservative Chromatography Clinical Biochemistry Pharmaceutical Science Salt (chemistry) Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry Hydroxybenzoate medicine Particle size Quantitative analysis (chemistry) medicine.drug |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 31:1060-1071 |
| ISSN: | 1520-572X 1082-6076 |
| DOI: | 10.1080/10826070801925532 |
| Popis: | The novel, rapid high performance liquid chromatographic method for the determination of ketoprofen lysine salt was developed and validated. The method can simultaneously assay methyl‐p‐hydroxybenzoate, used as a preservative in OKI spray. The separation was carried out on a C18 μBondapack (300×3.9 mm i.d., 10 µm particle size) column using acetonitrile—0.05 M KH2PO4 buffer (60∶40, v/v) as mobile phase (pH value 3.0 was adjusted with orthophosphoric acid) at a flow rate 1.5 mL min−1, temperature of the column 20°C, and UV detection at 254 nm. The method was found to be linear (r>0.9999) in the range of 8–24 µg mL−1 for ketoprofen lysine salt, and 7.5–2.5 µg mL−1 (r>0.9998) for methyl‐p‐hydroxybenzoate. The developed method was successfully applied to the determination of ketoprofen lysine salt, its investigated preservative in commercial formulation. The recovery for ketoprofen lysine salt and methyl‐p‐hydroxybenzoate were 100.2% and 100.4 with RSD of 0.6 and 0.7%, respectively. The method is rap... |
| Databáze: | OpenAIRE |
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