A less invasive system for the direct measurement of ventilation in fish
| Autor: | Junho Eom, Chris M. Wood |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences business.industry 030310 physiology Less invasive Aquatic Science 010603 evolutionary biology 01 natural sciences law.invention 03 medical and health sciences law Anesthesia Ventilation (architecture) Medicine Fish business GLUE Ecology Evolution Behavior and Systematics |
| Zdroj: | Canadian Journal of Fisheries and Aquatic Sciences. 77:1870-1877 |
| ISSN: | 1205-7533 0706-652X |
| Popis: | Most previous systems for quantifying ventilatory flow in fish involve prior anesthesia and difficult surgery to sew or glue membranes to the animal, which are undoubtedly stressful. By modification of the original “van Dam box” design and incorporation of an electromagnetic blood flow probe, we have developed a less invasive system that avoids these problems and provides breath-to-breath measurements of ventilatory flow in real time. The fish can be quickly moved in and out of the apparatus, facilitating repeated measurements on the same animal after different treatments. We have used the system to document the hyperventilatory and hypoventilatory responses to environmental hypoxia and hyperoxia, respectively, in both ∼400-g trout (Oncorhynchus mykiss) and 10-g goldfish (Carassius auratus); the method is easily adaptable to fish of other sizes. Separate experiments on trout have demonstrated that responses to these treatments in buccal pressure amplitude, breathing frequency, and ventilation index are not altered by the attachments used in the apparatus. This less invasive methodology may prove more acceptable to animal ethics committees. |
| Databáze: | OpenAIRE |
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