Genetic characterization of populations of the ectoparasitic caligid, Lepeophtheirus salmonis (Kroyer 1837) using randomly amplified polymorphic DNA

Autor:	Bryony A Dixon, Christina Sommerville, Andrew P. Shinn
Rok vydání:	2004
Předmět:	biology Ecology Dendrogram UPGMA Zoology Aquatic Science biology.organism_classification RAPD law.invention Salmon louse Lepeophtheirus law Genetic variability Salmo Polymerase chain reaction
Zdroj:	Aquaculture Research. 35:730-741
ISSN:	1365-2109 1355-557X
Popis:	Genetic variability within salmon louse, Lepeophtheirus salmonis (Caligidae: Copepoda), populations parasitizing farmed and wild Scottish Atlantic salmon (Salmo salar) was investigated using analysis of randomly ampli¢ed polymorphic DNA (RAPD) fragments. Seven individual decamer primers were used to analyse samples of salmon lice collected from 15 diierent locations in Scotland. The polymerase chain reaction products were separated using agarose gel electrophoresis and the resulting band patterns were analysed using a semi-automated analytical scoring system. Dendrograms were produced using the unweighted pair-group average (UPGMA) method using Dice similarity values. The summary dendrogram of the analysis of all RAPD bands showed two separate clusters of salmon lice, the larger being sub-divided into a further two sections. The collections of lice occupying each of these sub-divisions, however, were a mix of sites, which did not exhibit a structured geographical pattern.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2611e46d1007d448690fc4a6892446e3 https://doi.org/10.1111/j.1365-2109.2004.01095.x Zobrazit plný text záznamu