Isolation of Acidic Mucilage from the Outer Seed Coat of Shaddock (Citrus grandis Osbeck) and Evaluation of Its Functional Properties
| Autor: | Heng-Long Wang, Chen-Yi Lin, Chao-Lin Chang, Wan-Chi Wang, Wei-Yu Chen, Wei-Tung Liao, Li-Feng Chen, Yuan-Ting Hsu, Wen-Jen Yang, Pei-Jung Tsai, Wei-Zhi Wu |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Ruthenium red Quenching (fluorescence) DPPH 04 agricultural and veterinary sciences General Medicine 040401 food science 01 natural sciences digestive system diseases Staining Ferrous chemistry.chemical_compound 0404 agricultural biotechnology chemistry Mucilage Dry weight Biochemistry Imbibition 010606 plant biology & botany Nuclear chemistry |
| Zdroj: | American Journal of Plant Sciences. :780-788 |
| ISSN: | 2158-2750 2158-2742 |
| DOI: | 10.4236/ajps.2016.76072 |
| Popis: | After water imbibition, the outer layer seed coat of shaddock (Citrus grandis Osbeck) produces transparent gel-like mucilage (MSS), but its characteristics have never been studied before. This study aimed to assess the physico-chemical and functional properties of MSS. Extractions of MSS with deionized water at room temperature yielded about 3.5% based on the dry weight of seed. The major components were neutral sugars and uronic acids in the amounts of 33.5% and 49.6%, respectively. The acidic nature of MSS was confirmed by ruthenium red staining. Its water holding capacity and viscosity were 44.53 g•g-1 DW and 1660 cP at 10 g/L, respectively. MSS showed a weak quenching activity against DPPH radical, and moderate ferrous ion-chelating and superoxide anion radical scavenging activities, with IC50 value of 1.5 g/L and 1.1 g/L, respectively. A methyl thiazolyl tetrazolium (MTT) assay demonstrated that MSS significantly stimulated the viability of mouse skin fibroblasts (NIH/3T3) at 5 - 300 mg/L. These results impart the potential usefulness of the MSS to food, cosmetics and other applications. |
| Databáze: | OpenAIRE |
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