| Popis: |
The non-isotopic digoxigenin method provides a feasible alternative to the radioactive-system for the human VNTR determination. Initially, the digoxigenin probes were used in conjunction with colorimetric detection of DNA fragments [1, 2]. Then, it was shown that direct chemiluminescent detection enhanced the sensitivity. Furthermore, reprobing was easier with this technique since no colorimetric precipitate needed to be removed [1, 3, 4, 5]. On the other hand, direct chemiluminescence with AMPPD seemed to be more efficient and more flexible compared to luminol-based enhanced chemiluminescent system [6]. However the remaining problem of limited sensitivity is caused by the background from non specific binding of the anti-digoxigenin-alkaline phosphatase conjugate [7]. |
