Ultrasensitive Detection of Bovine Serum Albumin on DNA Modified Protein Microcapsules-based Electrodes
| Autor: | Dhananjayan Nathiya, Jeyaraj Wilson, Karuppasamy Gurunathan, Gokana Mohana Rani, Chikkili Venkateswara Raju, Vishal Chaudhary |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Journal of The Electrochemical Society. 170:057516 |
| ISSN: | 1945-7111 0013-4651 |
| DOI: | 10.1149/1945-7111/acd357 |
| Popis: | Bovine serum albumin (BSA) emerged as a potential bioprotein in the sensing field. Understanding the interactions of BSA with the analyte biomolecule has not been much reported. Herein, we report the functionally modified BSA (f-BSA) and its consecutive adsorption onto DNA for the ultrasensitive protein sensing. The DNA-modified f-BSA (DNA@f-BSA) composite was further characterized by using scanning electron microscopy, X-ray diffraction and Fourier transform-infrared spectroscopy. Obtained results demonstrated excellent composite formulation due to electrostatic interaction/binding intercalation and provided promising scaffold for protein biosensor. The as-prepared DNA modified f-BSA (DNA@f-BSA) based biocomposite can be used as a selective probe for the detection of BSA by recording square wave voltammetry signals. The sensitivity of the hybrid biosensor significantly improved the BSA detection over a wide concentration range from 1 × 10−20 g ml−1 to 1 × 10−4 g ml−1 with a detection limit of 2.18 × 10−21 g ml−1. Thus, the results have revealed the promising biosensor interactions which pave the way for the sensitive protein detection. |
| Databáze: | OpenAIRE |
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