Selective PF4 Release In Vitro Induced by Heparin and Related Glycosaminoglycans (GAGs) - Correlation with β-TG Release and Platelet Aggregation

Autor:	G Cristoforetti, Enrico Pogliani, Mariangela Colombi, Elisabetta Cofrancesco
Rok vydání:	1984
Předmět:	Glycosaminoglycan Aspirin Platelet aggregation Chemistry Heparin preparation medicine Platelet Human platelet Hematology Heparin Pharmacology In vitro medicine.drug
Zdroj:	Thrombosis and Haemostasis. 51:105-107
ISSN:	2567-689X 0340-6245
DOI:	10.1055/s-0038-1661032
Popis:	SummaryWe studied human platelet aggregation and β-TG/PF4 release induced by heparin and related GAGs in vitro both in normal PRP and in PRP after aspirin. In our experimental conditions, heparin and related GAGs always caused PF4 release in vitro from normal platelets, whether or not there was measurable platelet aggregation in the aggregometer. Significant β-TG release was induced only by the mucosal heparin preparation (which also induced platelet aggregation in some citrated PRP). Therefore, while β-TG release in vitro seems to correlate with platelet aggregating activity of heparin, the selective PF4 release, caused by heparin and related GAGs also in conditions in which neither platelet aggregation nor β-TG are measurable, is probably associated with the high affinity of PF4 for heparin. The degree of affinity of GAGs for PF4 (heparin > DeS > HS) seems to correlate with PF4 release. Moreover, the significant reduction in PF4 release in vitro after aspirin suggests that GAGs-induced PF4 release is related to a cyclooxygenase-dependent activation process.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::24ecd466cfd8780f984654d2522451b0 https://doi.org/10.1055/s-0038-1661032 Zobrazit plný text záznamu