A simple reversed-phase HPLC method for the estimation of dimethylarginines and homocysteine in plasma and serum
| Autor: | Venkatesh Ravula, Sridevi Annapurna Singh |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chromatography Homocysteine Phenyl isothiocyanate Coefficient of variation 010401 analytical chemistry Reversed-phase chromatography 01 natural sciences 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Sample preparation Derivatization Asymmetric dimethylarginine Sodium acetate |
| Zdroj: | Separation Science Plus. 1:404-410 |
| ISSN: | 2573-1815 |
| Popis: | Elevated concentrations of asymmetric dimethylarginine, symmetric dimethylarginine and homocysteine are considered as common risk factors for cardiovascular diseases. A simple reversed-phase high-performance liquid chromatography method has been developed to simultaneously detect these biomarkers in plasma and serum. Straightforward sample preparation involves direct derivatization of deproteinized serum/plasma with phenyl isothiocyanate. Dimethylarginines and homocysteine are resolved using a 250 × 4.6 mm I.D, C18 column and a linear gradient of sodium acetate buffer/acetonitrile. Calibration curves of peak area verses concentration are linear with correlation coefficients near unity. The intraday and interday precisions measured by coefficient of variation of peak areas were 1.34–9.7, 2.7–6.7 and 0.3– 7.1 for asymmetric dimethylarginine, symmetric dimethylarginine and homocysteine, respectively. The recoveries of asymmetric dimethylarginine, symmetric dimethylarginine and homocysteine are above 86%.Minimal sample preparation, good recoveries and fast run time makes this method suitable for the analysis of plasma and serum. |
| Databáze: | OpenAIRE |
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