Structured illumination super-resolution microscopy technology: review and prospect
| Autor: | 陈龙超 Chen Long-Chao, 李. 慧. Li Hui, 余. 佳. Yu Jia, 陈廷爱 Chen Ting-Ai, 高玉峰 Gao Yu-Feng, 郑. 炜. Zheng Wei |
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| Rok vydání: | 2018 |
| Předmět: |
Diffraction
Materials science Super-resolution microscopy Frequency band business.industry 0206 medical engineering Structured illumination microscopy 02 engineering and technology 020601 biomedical engineering Atomic and Molecular Physics and Optics Optics Optical transfer function Microscopy business Adaptive optics Biological imaging |
| Zdroj: | Chinese Optics. 11:307-328 |
| ISSN: | 2095-1531 |
| DOI: | 10.3788/co.20181103.0307 |
| Popis: | Structured illumination microscopy(SIM) is capable of providing super-resolution imaging. It breaks the diffraction limit by moving the high-frequency information of objects into the detectable frequency band of the optical imaging system via frequency mixing. Due to its attractive advantages, such as low intensity illumination, independence of particular fluorescent dyes, and rapid wide-field imaging capability, SIM has become the most popular technique for super-resolution imaging of living cells. This paper first systematically summarizes advances in the development of SIM and introduces corresponding principles at the same time. Then, two novel techniques of SIM developed by our group, including the single-photon excited super-resolution microscopy based on spectral unmixing and the two-photon excited super-resolution microscopy combined with adaptive optics, are particularly introduced in detail. At last, the recent applications and future directions of SIM in biological imaging are briefly discussed. |
| Databáze: | OpenAIRE |
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