Crotamine Cell-Penetrating Nanocarriers: Cancer-Targeting and Potential Biotechnological and/or Medical Applications
| Autor: | Brian Szychowski, Lucas Carvalho Porta, Eduardo B. Oliveira, Irina Kerkis, Mirian A. F. Hayashi, Joana D'Arc Campeiro, Richard L. Karpel, Marie-Christine Daniel, Wendel A. Alves |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell RNA law.invention Crotamine 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure chemistry Biochemistry law Colloidal gold 030220 oncology & carcinogenesis Recombinant DNA Nucleic acid medicine Nanocarriers DNA |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071603185 |
| DOI: | 10.1007/978-1-0716-0319-2_5 |
| Popis: | Crotamine is a basic, 42-residue polypeptide from snake venom that has been shown to possess cell-penetrating properties. Here we describe the preparation, purification, biochemical and biophysical analysis of venom-derived, recombinant, chemically synthesized, and fluorescent-labeled crotamine. We also describe the formation and characterization of crotamine-DNA and crotamine-RNA nanoparticles; and the delivery of these nanoparticles into cells and animals. Crotamine forms nanoparticles with a variety of DNA and RNA molecules, and crotamine-plasmid DNA nanoparticles are selectively delivered into actively proliferating cells in culture or in living organisms such as mice, Plasmodium, and worms. As such, these nanoparticles could form the basis for a nucleic acid drug-delivery system. We also describe here the design and characterization of crotamine-functionalized gold nanoparticles, and the delivery of these nanoparticles into cells. We also evaluated the viability of using the combination of crotamine with silica nanoparticles in animal models, aiming to provide slow delivery, and to decrease the crotamine doses needed for the biological effects. In addition, the efficacy of administering crotamine orally was also demonstrated. |
| Databáze: | OpenAIRE |
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