Autor: von Keller Angnes, Hans G.L. Coster, Tohsak L. Mahaworasilpa, Heide Schnabl
Rok vydání: 1998
Předmět:
Zdroj: Plant Cell, Tissue and Organ Culture. 55:59-62
ISSN: 0167-6857
DOI: 10.1023/a:1026480331508
Popis: The C4-directed enzymatic apparatus in guard cells is known to be coupled with a reduction in photorespiration resulting in a higher survival rate and yield potential of crops. This has led to searches for guard-cell-specific genes and promoters and methods for transferring them into C3-plants. To explore this possibility we performed somatic fusions between guard cell protoplasts from Vicia faba and hypocotyl protoplasts from Helianthus annuus, using a technique which allows fusion of a single pair of individually selected protoplasts. We obtained fusions in 30–70% of attempts. Culture of the hybrid fusion products in a liquid nutrient medium, without conditioning or feeder cells, produced microcolonies consisting of 8–9 cells within 9 days of culture.
Databáze: OpenAIRE
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