Induced Orientation of α-Helical Polypeptides in Polyelectrolyte Multilayers
| Autor: | and B. Kessler, Martin Müller, K. Lunkwitz |
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| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | The Journal of Physical Chemistry B. 107:8189-8197 |
| ISSN: | 1520-5207 1520-6106 |
| DOI: | 10.1021/jp027812x |
| Popis: | Polyelectrolyte multilayers (PEMs) consisting of stiff polypeptides at texturized silicon substrates were studied. These studies are focused on anisotropic PEMs in contrast with common ones consisting of flexible polyelectrolytes. When stiff polycation poly(l-lysine hydrobromide) (PLL, Mw = 200000 g/mol) was used at pH = 11 or in the presence of NaClO4, a transition from random coil (R−PLL) to α-helix (α-PLL) was found. When stiff polyanion poly(l-glutamic acid) (PLG, Mw = 70000) was chosen, the α-helical conformation (α-PLG) was adopted at pH = 3. The conformation was checked by IR spectroscopy and circular dichroism. The layer-by-layer technique was used in order to get homogeneous thin films of the polypeptides. Texturized Si-ATR-substrates with parallel nanosized grooves 50−70 nm in width were used for orienting the α-helical polypeptides with contour length L > 70 nm. Two different types of PEMs were fabricated: (i) α-PLG/poly(diallyldimethylammoniumchloride) (PDADMAC) and (ii) α-PLL/poly(vinyl sulf... |
| Databáze: | OpenAIRE |
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