Redox mediated biosensors incorporating cultured fish cells for toxicity assessment

Autor:	David M. Rawson, Monika E. Polak, Barry G. D. Haggett
Rok vydání:	1996
Předmět:	Biomedical Engineering Biophysics Biological activity General Medicine Metabolism Biology biology.organism_classification Redox Biochemistry Cell culture Toxicity Electrochemistry Fish Carp Biosensor Biotechnology
Zdroj:	Biosensors and Bioelectronics. 11:1253-1257
ISSN:	0956-5663
DOI:	10.1016/0956-5663(96)88090-0
Popis:	Fish are an important genus within ecosystems, and practical sensing devices, incorporating fish cells, could be used with advantage for environmental monitoring and protection. In this paper, redox mediated biosensors were prepared by immobilizing cultured fish cells at a carbon electrode surface. EPC (from carp) and BF-2 (from bluegill sunfish) cells could be monitored by using a lipophilic mediator (e.g. 2,6-dimethylbenzoquinone) added to the solution bathing the sensor. Currents measured in the external circuit were indicative of the metabolic activity of the immobilized cells and the sensors could be used to determine the presence of chemicals within the bathing medium that perturbed their normal metabolic status.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1de4c6504f0af52260691cc3f933a5aa https://doi.org/10.1016/0956-5663(96)88090-0 Zobrazit plný text záznamu Full Text from ScienceDirect