Quantification of Plasma and Leukocyte Vitamin C by High Performance Liquid Chromatography with Mass Spectrometric Detection

Autor:	Gabriella Donáth-Nagy, Szende Vancea, István Kiss, Attila Szőcs
Rok vydání:	2020
Předmět:	Chromatography Vitamin C Plasma samples Chemistry 010401 analytical chemistry Plasma 010402 general chemistry Ascorbic acid 01 natural sciences High-performance liquid chromatography Mass spectrometric Quantitative determination 0104 chemical sciences Analytical Chemistry Human plasma
Zdroj:	Journal of Analytical Chemistry. 75:1168-1176
ISSN:	1608-3199 1061-9348
DOI:	10.1134/s1061934820090038
Popis:	In the present study a high performance liquid chromatographic–mass spectrometric (LC–MS) method was developed for the measurement of ascorbic acid in human plasma and white blood cells. Special attention was paid to the known difficulties of ascorbic acid stability, and a plasma sample preparation method was developed, accordingly. The novel objective of this work was to focus on the determination of plasma ascorbic acid concentrations considering the necessity of accurate determination for further clinical studies related to scurvy induced by various pathological conditions. The presented study describes in detail a validated LC–MS method with adequate accuracy and precision for quantitative determination of ascorbic acid in human plasma. The method was tested on human plasma and leukocyte samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::1af008df77e3265b967d6aa7f7d093bf https://doi.org/10.1134/s1061934820090038 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.