Fractionation of cells for enzyme localization
| Autor: | J. D. Pollack, S. Razin, Robert C. Cleverdon, M.E. Pollack |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
chemistry.chemical_classification
Strain (chemistry) Membrane bound ATPase General Medicine Fractionation Mycoplasma Biology medicine.disease_cause Molecular biology General Biochemistry Genetics and Molecular Biology Cytolysis Enzyme Membrane chemistry Biochemistry biology.protein medicine General Pharmacology Toxicology and Pharmaceutics |
| Zdroj: | Life Sciences. 4:973-977 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(65)90200-6 |
| Popis: | Of six methods employed to disrupt cells of two Mycoplasma strains, only freeze-thaw and osmotic lysis permitted localization of NADH oxidase and ATPase activities. In one strain, NADH oxidase activity was confined to the soluble fraction, whereas it was in the membrane in the other; in both ATPase was largely membrane bound. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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