Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheat
| Autor: | T. Sumíková, L. Kucera, L. Gabrielová, J. Chrpová, M. Žabka |
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| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
0301 basic medicine Fusarium Czech Veterinary medicine 01 natural sciences law.invention 03 medical and health sciences chemistry.chemical_compound law Botany Genetic variability Mycotoxin Pathogen Polymerase chain reaction Chemotype biology food and beverages biology.organism_classification language.human_language 030104 developmental biology chemistry language Amplified fragment length polymorphism 010606 plant biology & botany Food Science |
| Zdroj: | Czech Journal of Food Sciences. 31:407-412 |
| ISSN: | 1805-9317 1212-1800 |
| DOI: | 10.17221/286/2012-cjfs |
| Popis: | Sumikova T., Gabrielova L., Kucera L., Žabka M., Chrpova J. (2013): Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheat. Czech J. Food Sci., 31: 407–412. Fusarium head blight (FHB) is a serious cereal disease in the Czech Republic. The most important pathogen associ ated with FBH is Fusarium graminearum , which can produce trichothecenes, mainly deoxynivalenol (DON) and its derivates. A set of 103 F. graminearum isolates were isolated from naturally infected wheat ears collected from 20 localities (25 ears from one locality) within the Czech Republic, in the year 2004. The ears were evaluated for DON content by ELISA. Maximum detected value was 30.7 mg/kg. A group of PCR assays targeting the segments of the Tri7 , Tri13 and Tri3 genes were used to determine the chemotypes of F. graminearum isolates. All the isolates belonged to DON producing chemotype. Further discrimination revealed that almost all (99.03%) isolates belonged to 15-ADON chemotype, and only one (0.97%) isolate belonged to 3-ADON chemotype. The genetic variability of the isolates was assessed from their AFLP fingerprints. The populations were highly heterogeneous both within and between locations, and no clear evidence for the association between AFLP profile and geographic origin was found out. |
| Databáze: | OpenAIRE |
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